Dimensional changes in boar spermatozoa during passage through the epididymis.

Changes in the surface of mouse spermatozoa during their passage through the epididymis

components, chymosins I and 11, by column chromatography on DEAE-cellulose (Fig. 1). The same characteristic profile was obtained from four different animals. Pooled fractions of chymosins I and I1 were examined by urea/polyacrylamide-gel electrophoresis, and each consisted of one major protein species, with chymosin I1 showing a slightly higher electrophoretic mobility than chymosin I. Chymosi...

A Study of the Function of the Epididymis Iii. Functional Changes Undergone by Spermatozoa during Their Passage through the Epididymis and Vas Deferens in the Guinea-pig

Kinematic changes during the cryopreservation of boar spermatozoa.

The present study evaluates the effect that various steps of a conventional cycle of cryopreservation have on the patterns of movement exhibited by boar spermatozoa. Sperm-rich ejaculate fractions collected from 24 mature fertile boars (1 ejaculate per boar) were cryopreserved following a standard freeze-thaw procedure with 0.5-mL plastic straws. Overall sperm motility and the individual kinema...

Changes in Mithun (Bos frontalis) spermatozoa during epididymal passage.

Genital organs of 10 healthy, adult Mithun bulls (6-8 years old) that were slaughtered at the dwellings of tribal people for meat were collected. Immediately after collection, spermatozoa from 3 different regions of the epididymis, i.e. the head, body and tail, were obtained to study morphological changes of the spermatozoa during passage through these regions. The prevalence of proximal cytopl...

Changes in plasma membrane glycoproteins of rat spermatozoa during maturation in the epididymis

Glycoproteins on the plasma membrane of testicular and cauda epididymidal spermatozoa have been labeled with galactose oxidase/NaB [3H]4 and sodium metaperiodate/NaB[3H]4, followed by analysis on SDS polyacrylamide gels. The major glycoprotein labeling on testicular spermatozoa has a molecular weight 110,000 whereas on cauda epididymidal spermatozoa greater than 90% of the radio-label is incorp...